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. 2014 May 2;9(5):e97208. doi: 10.1371/journal.pone.0097208

Figure 2. Effect of SKI-II on Nrf2 in primary human airway epithelial cells and in mice in vivo.

Figure 2

A. Whole-cell extracts from normal human bronchial epithelial cells (HBEC) treated with increasing concentrations of SKI-II (0.1 to 3 µM) for 2 h were analysed by immunoblotting for Nrf2 and normalized using β-actin. ** p<0.001, * p<0.05. B. Gene expression of NQO1, GCLM and HO-1 were determined after 24 h treatment with SKI-II (1 µM) in HBEC. GNB2L1 was used as housekeeping gene. *** p<0.0001, * p<0.05. C, D, E, F and G. A/J mice were exposed to nebulized SKI-II (10 µM in PBS, n  =  3) or vehicle (Veh, n  =  3) for 2 h followed by a further 2 h exposure. Whole-cell extracts were analysed by immunoblotting for Nrf2, GCLM, HO-1 and NQO1 and normalized using β-actin expression. Results are representative of 3 independent experiments and are means and S.E. of triplicates.