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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Cancer Res. 2014 Feb 3;74(9):2499–2509. doi: 10.1158/0008-5472.CAN-13-1531

Figure 3.

Figure 3

A) Endothelial cell migration assays were performed in triplicate for each cell line (UACC903-V1, UACC903-G2, and UACC903-G4) and repeated 3 times. HMVECnd migration is increased by the use of conditioned media from UACC903-G4 and UACC903-G3 cells compared to medium from control cells. Pictures taken with a Nikon eclipse TS100 inverted microscope with digital camera (Scale bar equals 100µM). B) Quantification of results shown in 3A (Average number of migrating cells ± SEM) (p<0.01). C) Angiogenic antibody array assay results show increased IL8 and VEGF secretion by cells with enhanced GRM1 expression compared to control cells. Row 1 (top row) is positive control. Row 5 dot 3 and 4 are IL-8, and Dot 7 and 8 are TIMP-1. Row 6 dot 1 and 2 are VEGF and dot 7 and 8 are TIMP-2. Validation of an increase in IL-8 (D, E, and F) and VEGF (G, H, and I) concentration in serum-free conditioned medium from UACC903-G2, UACC903-G4 (D and G), C8161-G21(E and H), C81-61-G6, and C81-61-G7 (F and I) cells compared to corresponding control cells (UACC903-V1, C8161-V2 C81-61-V3) using ELISA. Conditioned medium was diluted 20x for ELISA measurement. Experiments were repeated three times. Data presented are average ± SEM, p<0.001 for Figures 3 D-3I.