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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Cancer Res. 2014 Mar 3;74(9):2579–2590. doi: 10.1158/0008-5472.CAN-13-3470

Figure 2. Breast cancer cell lines have a differential sensitivity to autophagy inhibition.

Figure 2

A panel of breast cancer cell lines including 2 non-transformed (MCF10A, MCF12A), two luminal (MCF7, T47D), and four TNBC cell lines (two basal, HCC1937 and MDAMB468, and two claudin low, MDAMB231 and BT549) were transduced with shRNAs against ATG5, ATG7 or BECN1 and tested for clonogenic proliferation 8 days after plating. In A, cell lines were ordered according to their increasing sensitivity to autophagy inhibition as measured by crystal violet quantitation. Sample clonogenic images are shown. Alternatively, in B, live (trypan blue negative) cells were counted at different time points after plating and graphed in a proliferation curve. Graph in A shows mean ± SE of three independent experiments performed in triplicate. * = different from its respective non-silencing control; p < 0.01. In B, graphs show one representative experiment performed in triplicate.