Figure 2.

Comparison of CFM and CRM images at time points of interest. Images are from a representative 1.0 mg/ml collagen sample gelled at 32°C also shown in Video S1. Left panel: The first images collected, 88 s after neutralization. (a) The initial CFM image reveals small isotropic features of moderate intensity against a lower intensity homogeneous background, whereas (b) the earliest CRM image reveals no structures. Right panel: Images at arrest and plateau. (c) The CFM image at t_ARR (5.6 min) reveals many structures. (d) The CRM image at arrest displays structures of low intensity that can be better visualized when (e) contrast is enhanced. (f) The CRM image at plateau (12.5 min) reveals that many structures in the fully developed gel can already be visualized at arrest (as in (c) and (e)). Scale bar is 25 μm for all images.