Conformational properties of the free state of Gab2503-524 by analysis of CSs. (A) Populations of secondary-structure elements using δ2D (22,25). Black, orange, and green lines report the populations of helix, extended β, and PPII conformations, respectively. (B) Contents of random-coil regions estimated by δ2D (red) and RCI (26) (black line). The scale for the δ2D profile is reported on the right side (red) and the scale for RCI appears on the left side (black). To see this figure in color, go online.