Table 1.

ITC measurements of Grb2 SH3C interactions with WT and mutant peptides derived from Gab2

	Sequence	na	Kd (μM)	ΔH (kcal·mol−1)	−TΔS (kcal·mol−1)
WT	SRGSEIQPPPVNRNLKPDRKAK	0.91 ± 0.06	3.95 ± 0.51	−11.8 ± 1.33	4.46 ± 1.42
P510A	SRGSEIQAPPVNRNLKPDRKAK	1.11 ± 0.07	5.09 ± 0.34	−10.4 ± 0.94	3.15 ± 0.99
P511A	SRGSEIQPAPVNRNLKPDRKAK	–	TLQb	–	–
P512A	SRGSEIQPPAVNRNLKPDRKAK	0.99 ± 0.06	6.00 ± 0.25	−11.3 ± 0.34	4.22 ± 0.31
R515A	SRGSEIQPPPVNANLKPDRKAK	–	NBDc	–	–
K518A	SRGSEIQPPPVNRNLAPDRKAK	–	NBDc	–	–
P519A	SRGSEIQPPPVNRNLKADRKAK	0.84 ± 0.01	57.85 ± 1.7	−17.2 ± 0.36	11.4 ± 0.39

^aStoichiometry.

^bAffinity was too low for exact quantification.

^cNo binding was detected.