Figure 3.

Computer simulation of SHG from arrays of MTs. (A) SHG dependence on polarity at a density of 50 MTs/μm². Mean SHG increases quadratically with polarity, but with large variance. (B) SHG dependence on density, on a log-log scale, for MTs with antiparallel (green), parallel (red), and intermediate polarity (blue). Lines are simultaneous fits to the model given in Eq. 13. (C) SHG signal from MTs with the angular deviation from parallel drawn to a half-normal distribution, for MTs with antiparallel (green), parallel (red), and intermediate polarity (blue). SHG decreases at very high skew but is minimally affected at the level of angular disorder found in the spindle. Error bars in A–C show the standard deviation. (D) Histograms of simulated SHG at low (blue) and high (red) polarity. Distributions have positive skew but are close to Gaussian, more so at higher SHG signal. To see this figure in color, go online.