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. 2014 Apr 15;106(8):1607–1617. doi: 10.1016/j.bpj.2014.03.002

Figure 1.

Figure 1

The action spectra of channel currents (symbols and dashed lines in A, symbols and solid lines in B and C) generated in HEK293 cells by: (A) CaChR1 and its E169Q and D299N mutants; (B) WT DsChR1 and its A178E and A178E_E309Q mutants; (C) C1C2 and its E162Q and D292N mutants. Currents generated by the mutants of CaChR1 were measured at pH 5.4 because at pH 7.4 their amplitude was at the noise level. In all panels squares show the data from the pigments in which both photoactive site carboxylic acid residues are present, circles from the pigments in which the Asp-85 homolog is absent, and triangles from the pigments in which the Asp-212 homolog is absent. The solid lines in (A) show absorption spectra of detergent-purified WT CaChR1 and its E169Q and D299N mutants at pH 5.4. To see this figure in color, go online.