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Action spectra of channel currents generated in HEK293 cells by ChRs with the modified Lys-132 position and their carboxylic acid residue mutants: (A) CaChR1_F139K; (B) C1C2_K132F; (C) PsChR_K76F. In all panels squares show the data from the pigments in which both photoactive site carboxylic acid residues are present, circles in which the Asp-85 homolog is absent, and triangles in which the Asp-212 homolog is absent. To see this figure in color, go online.
