Comparison of the areal strain from 14 cells using the constant volume assumption, Sv, with the original data, So, which neglected a change in thickness. (Squares with error bars) Calculated Sv versus each measured S0 for living (solid squares) and ghost (open squares) cells. Here, for each cell, S0 is measured four times by using different start points for the snake algorithm under the same threshold as in Fig. 4 of Li et al. (1).