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. 2014 Apr 15;106(8):1559–1569. doi: 10.1016/j.bpj.2014.02.026

Figure 3.

Figure 3

PISEMA spectra from DgkA in liquid crystalline lipid bilayers of dimyristoyl-phosphatidylcholine and dimyristoyl phosphatidylglycerol in a 4:1 molar ratio. The molar ratio of lipid/protein was ∼200:1. (A) Spectra of uniform 15N-labeled protein. (B) Spectra of 15N tryptophan-labeled protein. (C) Spectra of 15N methionine-labeled protein. Because of the sensitivity of this experiment to 1H offset, to record the full spectrum, the 1H offset was set at two different frequencies for the UL 15N and 15N tryptophan-labeled samples (1). For these samples no functional assays were performed.