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. Author manuscript; available in PMC: 2014 May 3.
Published in final edited form as: J Biomol Screen. 2013 Nov 1;19(4):585–594. doi: 10.1177/1087057113510177

Fig. 1. Expression and purification of active Synj1 from HEK-239T cells.

Fig. 1

(A) HEK-293T cells stably expressing Synj1-GFP-FLAG labeled with indicated antibodies. Image was acquired with a 40x objective on an Olympus IX81 microscope using image analysis software Slidebook version 5.0. (B) Commassie stained gel showing aliquots from purification scheme of Synj1-GFP-FLAG. (C) Western blot detection of Synj1-GFP-FLAG using Synaptojanin 1 and FLAG specific antibodies. (D) Purified Synj1-GFP-FLAG phosphatase activity was detected using BODIPY labeled lipids and a positive control for lipid phosphatase activity, rat brain cytosol (RBC).