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. Author manuscript; available in PMC: 2014 May 3.
Published in final edited form as: J Biomol Screen. 2013 Nov 1;19(4):585–594. doi: 10.1177/1087057113510177

Fig. 3. Lipid phosphatase assay using SHIP2.

Fig. 3

(A) SHIP2 phosphatase activity with 120 μM preferred substrate PI(3,4,5)P3 compared to minimal activity toward 120 μM PI(4,5)P2 with respect to time. Specific activity in relative fluorescent units is shown for which background fluorescence has been subtracted at each time point. (B) Total activity of SHIP2 toward 120 μM PI(3,4,5)P3 and background fluorescence of reaction without enzyme (no Enz). (C) Specific activity with respect to increasing concentration of lipid substrate at 100 minutes after assay start. (D) SHIP2 and Synj1 activity in presence of AS1949490 shown as % SHIP2 activation after normalization to phosphate standard curve and calculation of phosphate liberated per minute (μM/min). Error bars represent standard error of the mean.