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. Author manuscript; available in PMC: 2014 May 3.
Published in final edited form as: J Biomol Screen. 2013 Nov 1;19(4):585–594. doi: 10.1177/1087057113510177

Fig. 4. Optimization of Synj1 activity assay.

Fig. 4

(A) Z′ factor calculated for each time point for specific activity of 100 ng Synj1 at differing lipid concentrations. (B) Signal to Background (S:B) is shown for Synj1 activity compared to background fluorescence in control without enzyme (C) % CV is shown for variance of Synj1 activity compared to specific Synj1 activity. Results are representative of at least two independent experiments.