Skip to main content
. 2014 Apr 24;176(3):351–362. doi: 10.1111/cei.12291

Figure 5.

Figure 5

Interferons (IFNs) and polyinosine-polycytidylic acid [poly (I : C)] stimulates 21OH mRNA expression in H295R cells. Total RNA was isolated from H295R cells following 24 h stimulation with IFNs or poly (I : C). 21OH expression was measured by reverse transcription–quantitative polymerase chain reaction (RT–qPCR) and normalized to β-actin. Results are expressed as mean fold induction over unstimulated cells (medium) ± standard deviation (s.d.) of three independent experiments. Asterisks indicate statistical significance versus unstimulated (medium) cells (**P < 0·01; ***P < 0·001) as determined by one-way analysis of variance (anova) followed by Dunnett's post-hoc test.