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. 2014 Apr 28;171(10):2608–2620. doi: 10.1111/bph.12320

Figure 2.

Figure 2

Dose-dependent effects of (±) evodiamine; S(+) evodiamine and R(−) evodiamine on TRPV1-mediated elevation of intracellular calcium in HEK-293 cells stably overexpressing the human (A) or the rat (B) recombinant TRPV1 channel. Data are means of n = 6 separate experiments. The specificity of the receptor response was also verified by pretreating the cells transfected with TRPV1 for 5 min with the specific antagonist 5-iodo-resiniferatoxin (IRTX) (Wahl et al., 2001) at a concentration of 10 nM before the addition of the compound at the concentration of 1 μM. This resulted in a complete inhibition of the activity of all three evodiamines at TRPV1 (empty circle).