Figure 5.

Western blot analysis for TRAP after chronic treatment with palvanil in sham and OVX mice in vivo: Western blot analysis and relative quantification of TRAP expression in homogenized murine femur normalized with respect to β-tubulin. A significant increase in TRAP levels in bone from OVX mice with respect to sham animals was found. Chronic treatment with palvanil 1 mg·kg⁻¹ increased TRAP levels in bone dissected from sham mice, while reduced them in bone from OVX mice. Data are presented as a mean ± SD. Differences in the mean values were evaluated by Student's unpaired t-test. P < 0.05 was considered statistically significant.