Figure 7.

TRAP levels decrease in bone tissue from old mice. (A) TRAP decreases in bone tissue from Trpv1^−/− mice as compared with that from WT mice. Ageing significantly reduced TRAP levels. The genetic ablation of Trpv1 did not exert any significant change in bone tissue from old mice. (B) Western blot analysis and relative quantification for TRAP expression in homogenized murine femur normalized with respect to β-tubulin. A significant decrease in TRAP levels was observed in bone from Trpv1^−/− young animals, as well as in old bone tissue. (C) CB₂ receptor levels were increased in bone tissue from Trpv1^−/− mice as compared with that from WT, both in young and old animals. Ageing dramatically reduced CB₂ receptor levels. (D) TRPV1 mRNA levels were significantly reduced in bone tissue from old mice.