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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Nat Cell Biol. 2014 Apr 6;16(5):425–433. doi: 10.1038/ncb2935

Figure 2.

Figure 2

The C-terminus of Atg19 interacts in a cargo-dependent manner with Atg8

(a) Anti-Atg8 western blot of the input and pellet fractions of a pull down experiment using GST-Atg19 as bait and Atg8 as prey. The black vertical line indicates that unrelated samples were run on the same gel but in between the two samples on the left and the one on the right (see Supplementary Fig. 7). (b) Coomassie stained gels of pull down experiments using the GST-prApe1 propeptide as bait to pull down Atg19, the Atg19-Atg34 chimera and Atg34, respectively. The Atg19-Atg34 chimera contained residues 1-361 of Atg19 and residues 348-412 of Atg34. (c) Yeast-two-hybrid assay testing for the interaction of Atg11 with Atg19, the Atg19-Atg34 chimera and Atg34, respectively. (d) Anti-Atg8 western blot using the indicated GST fusion proteins as bait to pull down Atg8. The pull downs were conducted in the presence or absence of the prApe1 propeptide. See Supplementary Fig. 1 for input gel. All experiments shown have been done two times. Images of uncropped western blots and gels can be found in Supplementary Figure 7.