Fig. 7.

Response of two insilico FPS1 mutants to 0.5 M NaCl shock applied at t = 0 min. Mutants are (1) ΔFPS1: Mutant in which PTP and glycerol feedbacks are present but FPS1 is not present so there is very less leakage of glycerol (dashed line). (2) Mutant in which FPS1 channel is not regulated by turgor pressure, thereby, the glycerol transport rate is not sensitive to the turgor pressure (dash-dotted line). Solid line represents wildtype response. a Profiles of deviation of Hog1PP (i.e. deviation from pre-shock level in wild type) normalised by the maximum deviation obtained in wild type. b Profiles of Enzyme, normalised by pre-shock level in wild type. c Normalised error in turgor pressure as defined in Eq. 1. d Profile of intracellular glycerol accumulation, normalised by pre-shock level in the wild type