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The cytological and immunocytochemical evaluation of spermatogonial stem cells using PGP9.5 immediately after isolation. The spermatogonia were seen as different forms: single (arrow, A, B), paired (arrowhead, A, B) aligned (triangle, A, B) and cluster (asterisk, A, B). The isolated SSCs were round cells with a spherical nucleus, a high nucleus, cytoplasm ratio, and many cytoplasmic inclusions that tended to congregate and form small cell clusters
