Figure 4.

Immunocytochemical evaluation of differentiated type A spermatogonia in percoll separation method using ckit antibody. The proportion of c-kit positive cells (brown cells) in A) 32%; B) 30%; C) 28% and D) 20% gradients were determined. The highest (D) and lowest (A) percentages of differentiated spermatogonia were found in gradients 32 and 20% respectively. Note that the strong and weak c-kit immunoreactivity was observed among the aggregated (Aal) and committed (A1-A4) spermatogonia, respectively. E) Negative and F) positive controls were detected