Table 1.
Two methods of goat SSCs purification using PGP9.5 and c-kit molecular markers
| Molecular marker | Purification method | |||||||
|---|---|---|---|---|---|---|---|---|
| Percoll | Differential plating | |||||||
| 20% | 28% | 30% | 32% | 3 hr | 4 hr | 5 hr | 6 hr | |
| PGP 9.5 (%) | 41.5±3.1a | 85.7±1.2b | 92.7±0.6b,c | 94.6±0.4c,A | 55.5±1.1a | 62.9±0.8b | 81.3±1.1c,B | 74.9±1.3d |
| c-kit (%) | 63.0±0.7a | 33.5±0.7b | 18.1±0.9c | 25.1±0.7d,A | 43.6±1.3a | 27.3±0.9b | 17.1±1.4c,B | 26.3±1.2b |
a-d
In each method numbers with different lowercase letters in the same row differ significantly (p < 0.001)
A, B
Numbers with different uppercase letters in the same column indicates significant difference between two methods (p < 0.001)