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. 2014 Apr 14;2014:209163. doi: 10.1155/2014/209163

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Copyright © 2014 Zhaona Wu et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Representative fluorescence micrographs showing apoptosis of Caco-2 cells after DAPI staining. Cells were grown on glass coverslips and incubated for 24 h with culture media, ST-4, ST-7, or 0.5 μM staurosporine as positive control. Cells coincubated with ST-7 and staurosporine exhibit nuclear fragmentation and condensation (arrow) typical of apoptotic cells. Histogram represents percentage of apoptotic cells after DAPI fluorescence assay. Caco-2 monolayers coincubated with ST-7 exhibited significantly higher percentage of apoptosis (P value < 0.05) compared to ST-4 and negative control. Values are means ± standard error (n = 6 per group). For each sample, ~100 cells were counted at 1000x magnification.