Figure 1.

Biofilm localization in shaking 96-well microtiter plates placed in a 50 mm incubator at 150 rpm. (a) Photograph of a well stained with crystal violet; (b) schematic representation of a well where the dark grey area corresponds to the wetted area without shaking, the light grey area represents the area increase upon shaking, and the dotted line depicts the inclination of the air-liquid interface; (c) time averaged shear strain rates (values below 20 s⁻¹ are not represented); (d) illustration of the biofilm distribution on the vertical wall assayed by the differential CV staining; (e) representative scanning electron micrographs of the wall sections defined in image (d); (5000x magnification bar = 10 μm).