Table 2.
Probe set IDa | Gene name | Signals/encoding enzymes | Microarray hybridization signalsb | Fold changesc | |||
---|---|---|---|---|---|---|---|
0 | 0.01 mM | 5 mM | 0.01 mM | 5 mM | |||
Average of all probe sets | 554.2 ± 2.3 | 557.0 ± 1.9 | 560.2 ± 1.8 | ||||
| |||||||
1762328 | glmM | Phosphoglucosamine mutase | 61.0 ± 15.4 | 64.3 ± 6.8 | 32.0 ± 4.7 | 1.1 | −1.9 |
1764010 | rfaP | Lipopolysaccharide core biosynthesis protein rfaP | 33.7 ± 1.7 | 51.8 ± 9.5 | 49.4 ± 1.6 | 1.5 | 1.5 |
1764732 | yegB | Multidrug efflux system protein MdtE | 27.0 ± 10.5 | 55.4 ± 3.7 | 48.9 ± 16 | 2.2 | 1.8 |
1767270 | ydbA | Hypothetical protein | 144.8 ± 17.6 | 90.4 ± 3.8 | 135.3 ± 18.2 | −1.6 | 1.1 |
1768816 | ynbC | Hypothetical protein | 55.5 ± 4.6 | 32.0 ± 9 | 42.6 ± 6.1 | −1.8 | 1.3 |
1768993 | phnL | PhnI protein | 50.6 ± 10.1 | 70.1 ± 14.2 | 90.7 ± 15 | 1.4 | 1.8 |
1768842 | nfi | Endonuclease V | 43.9 ± 6.3 | 72.1 ± 15 | 43.3 ± 11.3 | 1.6 | 1.0 |
aSelected probe sets corresponding to selenite-responsive candidate genes have absolute fold changes ≥1.5 and t-test P values ≤ 0.05 either in 0.01 or 5 mM Na2SeO3 treatment. bHybridization signals of average of all 10,208 probe sets and seven probe sets. Mean values ± SD (n = 3). Average of background signals are 39.7 ± 2.3, 45.8 ± 1, and 47.4 ± 0.4 for 0, 0.01 and 5 mM, respectively. cFold changes of selenite-responsive candidate genes from microarray analysis comparing to untreated control. “−” means reduced expression.