Table 5.
Comparison of nucleotide sequences and abundance of sequenced DGGE bands.
| Band | Sequence | Accession no. | Closest relative (accession no.) | Identity | Relative abundance of bands (%) | ||
|---|---|---|---|---|---|---|---|
| (bp) | (%) | 1# | 2# | 3# | |||
| 4 | 188 | KF234427 | Uncultured bacterium clone ASNR-16 (JQ809244) | 100 | 7.0 | 2.3 | 4.2 |
| 8 | 188 | KF234428 | Uncultured Chlorobi bacterium clone ABL17 (JQ906960) | 97 | 1.3 | 1.9 | 8.4 |
| 10 | 183 | KF234429 | Uncultured Nitrospira sp. (KC491391) | 100 | 11.5 | 6.7 | ND |
| 11 | 193 | KF234430 | Uncultured Nitrosomonas sp. clone S12 (AY605680) | 99 | 3.5 | 3.7 | ND |
| 12 | 187 | KF234431 | Flavobacterium sp. R046 (KC252875) | 99 | ND | ND | 7.1 |
| 13 | 188 | KF273869 | Uncultured Bacteroidetes bacterium (FJ750465) | 100 | 1.4 | 6.8 | ND |
| 14 | 193 | KF234435 | Uncultured beta proteobacterium (AB636023) | 100 | ND | ND | 5.7 |
| 16 | 193 | KF234436 | Uncultured Aquabacterium sp. (JQ288705) | 99 | ND | ND | 12.8 |
| 18 | 193 | KF234437 | Leptothrix sp. (JQ946011) | 100 | ND | ND | 5.7 |
| 23 | 170 | KF234438 | Uncultured Alphaproteobacteriabacterium (CU925173) | 100 | ND | ND | 1.5 |
| 25 | 193 | KF234441 | Uncultured bacterium (KC541099) | 98 | ND | ND | 3.6 |
| 26 | 193 | KF234444 | Thauera sp. enrichment culture clone LDC-8 (KF020718) | 100 | 8.4 | 9.2 | 10.6 |
| 28 | 196 | KF234446 | Uncultured proteobacterium (GQ243004) | 98 | ND | ND | 2.6 |
ND: not detected.
The bands are designed as shown in Figure 4.
The abundance was calculated using BandScan (version 5.0) software.