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. 2014 Apr 15;2014:582730. doi: 10.1155/2014/582730

Figure 6.

Figure 6

TanIIA induced esophageal cancer cells apoptosis through Akt/Bax/Bcl-2/caspase-9/caspase-3 pathway and arrested cell cycle in S and G2/M phase through p53/p21/CDC2 and cyclin B1 complex pathway. Total cell lysates were prepared and quantified by BCA protein assay kit. Western blots were detected with antibodies against Akt1, phospho-Akt1, p53, Bax, Bcl-2, procaspase-9, cleaved-caspase-9, caspase-3, caspase-3 active, p21, CDC2, phospho-CDC2, cyclin B1, and beta-tubulin. A representative blot is shown by independent experiments in triplicate.