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. Author manuscript; available in PMC: 2014 Jun 15.
Published in final edited form as: J Neurosci Methods. 2013 Apr 3;216(2):104–109. doi: 10.1016/j.jneumeth.2013.03.020

Figure 2. The High-Low oxygen condition does not impact cell proliferation and survival and can be mimicked by inhibitors of GSK3β and SMAD signaling pathways.

Figure 2

(A) Cartoon representation of the experimental design. (B) Ki67 quantification shows no significant difference between the High-High and the high-low conditions. Bar graph represents the percentage of Ki67 positive cells (n=4). (C) Bar graph showing average number of Dapi staining per 40x field of view (n=4) showing similar numbers of cells in the High-High and the High-Low conditions. (D) Ki67 and Ascl1 immunoflurescent staining showing no overlap after doxycycline. (E) Based on (Ladewig et al., 2012) inhibitors of GSK3β and SMAD signaling pathways were tested for synergistic interaction with low O2 reprogramming condition. Bar graph represents fold increase of Map2 positive cells from two independent experiments. No apparent synergistic effect was apparent.