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. 2014 Apr 28;8:40. doi: 10.3389/fncir.2014.00040

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Copyright © 2014 Huma, Du Beau, Brown and Maxwell.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confocal images of double-labeled cells in the pons. (A) Shows a montage of a coronal section through the pons of an animal that had received a MLF and spinal injection, (both on the right side). Areas 1 and 2 within the boxes are shown at higher magnification in series (B,C) respectively which are short projected sequences of confocal images showing cells labeled from the MLF (B,C), the spinal cord (B′,C′) and merged images showing yellow double-labeled cells (B″,C″). 4V, 4th ventricle; LDTg, laterodorsal tegmental nucleus, mcp, middle cerebellar peduncle, Pn, pontine nuclei, PnO, pontine reticular nucleus, oral part. Arrows indicate Double- labeled cells. Scale bars = 50 μm.