Table 2.
Antibodies used in the study.
| Primary antibody combination | Primary antibody concentration | Supplier | Secondary antibodies | |
|---|---|---|---|---|
| A | gt. CTb | 1:5000 | List Biological Laboratories, Campell, CA | Rh.Red |
| rbt FG | 1:5000 | Chemicon/Millipore,CA,USA | Alexa488 | |
| B | mo. CTb | 1:250 | A. Wikström, University of Gothenburg | Rh.Red |
| gp VGLUT1 | 1:5000 | Millipore, Harlow, UK | Alexa488 | |
| gp VGLUT2 | 1:5000 | Millipore, Harlow, UK | Dylight 649 | |
| C | mo. CTb | 1:250 | A. Wikström, University of Gothenburg | Rh.Red |
| rbt VGAT | 1:1000 | Synaptic System, Göttingen, Germany | Alexa488 | |
| gp VGLUT1 | 1:5000 | Millipore, Harlow, UK | Dylight 649 | |
| gp VGLUT2 | 1:5000 | Millipore, Harlow, UK | Dylight 649 | |
| D | gt. CTb | 1:5000 | List Biological Laboratories, Campell, CA | Rh.Red |
| rbt GLYT2 | 1:1000 | Millipore, Harlow, UK | Alexa488 | |
| mo GAD67 | 1:1000 | Millipore, Harlow, UK | Dylight 649 | |
| E | mo. CTb | 1:250 | A. Wikström, University of Gothenburg | Rh.Red |
| rbt Serotonin | 1:100 | Affiniti, Exeter, UK | Alexa488 |
gt, goat; rbt, rabbit; mo, mouse; gp, guinea pig; FG, Fluorogold; CTb, the b subunit of cholera toxin; VGLUT1, vesicular glutamate transporter 1; VGLUT2, vesicular glutamate transporter 2; VGAT, vesicular GABA transporter; GLYT2, glycine transporter 2; GAD67, 67 isoform of glutamate decarboxylase. In Experiment C tissue was incubated in a mixture of VGLUT1+2 antibodies.