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. Author manuscript; available in PMC: 2014 May 5.
Published in final edited form as: Adv Healthc Mater. 2013 Apr 8;2(10):1401–1410. doi: 10.1002/adhm.201300006

Figure 5. Morphological and migratory changes to dendritic cells following vaccine delivery.

Figure 5

Immunohistochemical study of LCs in human skin following microneedle delivery of H1N1 VLPs. A) Epidermal sheets isolated from skin 24 h after placebo (Blank coat) and VLP (VLP MN) delivery (Bar = 100μm). B, C) Immunohistochemically stained longitudinal sections of human skin treated with (B) VLP and (C) placebo (Bar = 200μm). D) Immuno-stained epidermal sheets were visualised by light microscopy 2, 8 and 24 h after microneedle delivery of H1N1 VLPs. The number of CD207+ cells observed in 200μm2 fields of view are shown for VLP treated samples (black circles) and placebo treated samples (white circles). A total of 3-5 replicate counts were taken from a total of 4 independent skin donors. ***denotes significant (P<0.01) difference between VLP and control samples at each timepoint.