Effect of CBDA on the horizontal migration of MDA-MB-231 cells. (A) Wound-healing assays (horizontal migration) were performed to determine the effects of CBDA or CBD on MDA-MB-231 cell migration. (a) Representative images of the migrating cells were captured 12 h after vehicle (indicated as Cont.), 5 μM or 25 μM CBDA and 5 μM CBD treatments. (b) Migration data presented in panel (a) was assessed on the basis of percent wounded area filled in. Data are expressed as the percent of vehicle-treated group (indicated as Cont.), as mean ± S.D. (n = 8). (B) Migration data was assessed on the basis of percent wounded area filled in 12, 24, or 48 h after treatments with 25 μM CBDA. Data are expressed as the percent of vehicle-treated group (indicated as Cont.), as mean ± S.D. (n = 8) *Significantly different (p < 0.05) from the respective vehicle-treated controls.