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. Author manuscript; available in PMC: 2014 May 5.
Published in final edited form as: Toxicol Lett. 2012 Sep 8;214(3):314–319. doi: 10.1016/j.toxlet.2012.08.029

Fig. 5.

Fig. 5

CBDA stimulates RhoA activity. (A) RhoA affinity pull-down assays were used to determine the level of active RhoA according to the methods described in Section 2. Resulted pull-down samples were subjected to Western blot analyses using an anti-RhoA antibody. Active RhoA was increased by 25 μM CBDA (indicated as +) in a time-dependent manner. Western blot analyses were also performed using an anti-RhoA antibody specific to RhoA phosphorylated at Ser188 and a β-actin antibody. (B) Results are expressed as the ratio of active RhoA to total RhoA protein in each cell lysate. Data are expressed as the fold change vs. vehicle-treated group (indicated as −), as mean ± S.D. (n = 3). *Significantly different (p < 0.05) from the vehicle-treated control.