Table II.
Maturation rates of cultured pre-antral follicles derived from fresh ovaries, vitrified ovaries and vitrified follicles in the presence or absence of alpha lipoic acid (ALA)
| Groups | ALA | N | Survived | Degenerated | Antrum formation |
Maturation stages of oocyte
|
||
|---|---|---|---|---|---|---|---|---|
| GV | MI | MII | ||||||
| Fresh ovaries | ||||||||
| - | 240 | 204a (85.0±1.9) | 36a (15.0±1.9) | 184a (76.7±5.6) | 30 (12.5±3.5) | 51 (21.2±3.7) | 103a (42.9±2.8) | |
| + | 180 | 169 b (94.3±1.2) | 11b (5.7±1.2) | 166b (92.3±4.0) | 16 (9.5±2.4) | 34 (18.9±5.1) | 97b (53.9±4.2) | |
| Vitrified ovaries | ||||||||
| - | 180 | 102c (57.3±3.5) | 72c (43.3±2.9) | 83c (46.0±4.2) | 27 (15.0±1.7) | 22 (12.2±3.5) | 27c (15.0±1.7) | |
| + | 180 | 120d (66.7±3.3) | 60d (33.3±3.3) | 111d (60.6±2.5) | 20 (11.1±2.5) | 31 (20.6±4.2) | 48d (26.7±1.7) | |
| Vitrified pre-antral follicle | ||||||||
| - | 180 | 123 d (68.3±6.0) | 57d (31.7±6.0) | 113d (62.8±4.2) | 21 (11.7±1.7) | 20 (11.1±2.5) | 54d (28.3±1.7) | |
| + | 180 | 150a (83.3±3.3) | 30a (16.7±3.3) | 134a (74.4±4.2) | 20 (11.1±2.5) | 21 (11.7±1.7) | 66a (36.7±3.3) | |
Data were presented as number (% ± SD) in all columns.
In all cases at least 4 experimental replicates were performed.
Different superscripts in the same column reflect different levels of significant difference (p<0.05)
GV: germinal vesicle stage oocyte; MI: metaphase I oocyte; MII: metaphase II oocyte.