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. 2014 May;24(5):724–732. doi: 10.1101/gr.170167.113

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© 2014 Baker et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 3. — NDR length is dependent on H3K4me3 level. (A) Two examples of calculating the NDR at individual hotspots, one activated by each Prdm9 allele (red: Prdm9^Cst; blue: Prdm9^Dom2). Hotspot nucleosome positions are indicated below the coverage profile with filled bars and PRDM9 binding motif positions in black. The NDR length was determined by subtracting 150 from the distance (d) between the centers of the −1 and +1 nucleosomes. (B) Distribution of NDR sizes for Prdm9^Dom2 hotspots (n = 5538). (C) Distribution of NDR sizes for Prdm9^Cst hotspots (n = 9194). (D) Relationship between H3K4me3 level and NDR length at Prdm9^Dom2 hotspots (error bars: standard error of the mean NDR length for each H3K4me3 category). (E) Relationship between NDR length and H3K4me3 level at Prdm9^Cst hotspots.