Figure 1.

Steps required for attaining cell type-specific transcriptomes. (1) Microinject a group of zygotes with an artificial chromosome genetically engineered to express a fluorescent reporter in the cell type of interest; (2) culture transgenic zygotes until they have reached the desired developmental stage, then disaggregate the embryos to individual cells; (3) run the cells through flow cytometry in order to segregate populations of cells by way of FACS; and (4) isolate, amplify, sequence, and quantify the pool of mRNA from each population in order to calculate relative enrichment for each gene. (AU) Arbitrary units; (BACs) bacterial artificial chromosomes.