Fig. 8.
Effect of NF-κB inhibitor CAPE on IL-1β-induced activation of the NF-κB promoter (a) and MIP-1α and -1β expression (b) in NT2-N. (a) NT2-N cells were transfected with plasmid containing the NF-κB promoter (pNF-κB-luc). The cells were then incubated with or without IL-1β and/or CAPE for 12 h. Data are mean ± SD of triplicate cultures, representative of three independent experiments. (b) NT2-N cells were preincubated with or without CAPE (25 μg/mL) for 2 h. The cells were then treated with IL-1β (4 ng/mL) for 6 h. Total cellular RNA was extracted and subjected to real-time RT–PCR to detect MIP-1α and -1β mRNA expression. Data are mean ± SD of triplicate cultures, representative of two independent experiments.