Skip to main content
. Author manuscript; available in PMC: 2014 May 5.
Published in final edited form as: J Infect Dis. 2001 Dec 14;185(1):118–122. doi: 10.1086/338011

Figure 3.

Figure 3

Effect of methadone on human immunodeficiency virus (HIV) replication in peripheral blood mononuclear cells (PBMC) from 6 HIV-infected patients. CD8 T cell–depleted PBMC were stimulated with 1% phytohemagglutinin (vol/vol) for 72 h. Cells then were incubated with or without methadone (10−10 M) and/or anti-CD3 (2 μg/mL), as indicated above. In patient 4, morphine (10−10 M) was also tested in an HIV induction experiment. Cultures were refed with fresh medium containing the indicated reagents every 3 days. Day 6 culture supernatants were collected for HIV reverse-transcriptase (RT) assay. Data are mean ± SD of HIV RT activity in triplicate cultures. Plus (+) and minus (−) signs indicate presence or absence.