A. Affymetrix APRIL, IGF-1, IL-10, BAFF and IL-6 gene expression in BM CD34 cells (n = 5), BM stromal cells (n = 5), purified BM CD15 (n = 5), CD14 (n = 5) and CD3 cells (n = 5), osteoclasts (n = 7), normal memory B cells (n = 6), normal polyclonal plasmablasts (n = 7), normal BM plasma cells (BMPC) (n = 7), and purified myeloma cells from patients with multiple myeloma (MM) (n = 131).
B. The concentrations of BAFF, APRIL, IL-10, IL-6, or IGF-1 were assayed with an ELISA in 3-day culture supernatant of XG-1 and XG19 cells, osteoclasts and XG-1/osteoclasts and XG-19/osteoclasts cocultures. Results are the mean value of three independent experiments.