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. Author manuscript; available in PMC: 2014 May 6.
Published in final edited form as: Blood. 2010 Nov 19;117(4):1280–1290. doi: 10.1182/blood-2010-04-279760

Figure 5. Osteoclasts promote the survival of cytokine-dependent myeloma cell lines.

Figure 5

Figure 5

A. XG-19 and XG-20 myeloma cells were cultured at 105 cells/ml without cytokine, or with IL-6 (2 ng/ml), or with 104 osteoclasts with or without anti-IL-6 (BE8) MoAb (10 μg/ml), TACI-Fc (20 μg/ml), anti-IGF-1R MoAb (4 μg/ml), anti-IL-10 MoAb (10 μg/ml) or anti-CCR2 MoAb (10 μg/ml). Cells were recovered after 3 days of culture and apoptotic cells were detected by annexin V staining. Results are those of one experiment representative of five.

B. Purified MMC of patients were cultured at 105 cells/ml without cytokine or in the presence of osteoclasts (1 osteoclast for 4 MMC) or IL-6 (2 ng/ml). Cells were recovered after 2, 5 and 6 days of culture and apoptotic cells were detected by annexin V staining.