. Author manuscript; available in PMC: 2015 Feb 1.

Published in final edited form as: Biomed Chromatogr. 2013 Sep 10;28(2):281–294. doi: 10.1002/bmc.3019

Table 5.

Peak assignment for the analysis of compounds using UHPLC-MS Method

Peak	RT	(+)-ESI-MS (m/z) [M+H]⁺	MS Fragment ions (m/z)	(−)-ESI-MS (m/z) [M-H]⁻	Identities
1	2.79	1209.5 [M+H-H₂O]⁺	1063.5 [M+H-H₂O-Rha]⁺ 901.5 [M+H-H₂O-Rha-Glu]⁺ 739.4 [M+H-H₂O-Rha-2Glu]⁺ 577.4 [M+H-H₂O-Rha-3Glu]⁺ 415.3 [M+H-H₂O-Rha-4Glu]⁺	1225.6	parvifloside
2	2.95	1047.5 [M+H-H₂O]⁺	901.5 [M+H-H₂O-Rha]⁺ 739.4 [M+H-H₂O-Rha-glu]⁺ 577.4 [M+H-H₂O-Rha-2glu]⁺	1063.5	protodeltonin
3	3.11	1031.5 [M+H-H₂O]⁺	885.5 [M+H-H₂O-rha]⁺ 739.4 [M+H-H₂O-2Rha]⁺ 577.4 [M+H-H₂O-2Rha-Glu]⁺ 415.3 [M+H-H₂O-2Rha-2Glu]⁺	1047.5	protodioscin
4	3.36	885.5 [M+H-H₂O]⁺	739.4 [M+H-H₂O-Rha]⁺ 577.4 [M+H-H₂O-Rha-Glu]⁺ 415.3 [M+H-H₂O-Rha-2Glu]⁺	901.5	protobioside
5	4.95	1047.5	901.5 [M+H-Rha]⁺ 739.4 [M+H-Rha-Glu]⁺ 577.4 [M+H-2Glu-Rha]⁺ 415.3 [M+H-3Glu-Rha]⁺ 397.3 [M+H-3Glu-Rha-H₂O]⁺	1045.5	huangjiangsu A
6	5.12	1031.5	885.5 [M+H-Glu]⁺ 739.4 [M+H-Glu-Rha]⁺ 577.4 [M+H-2Glu-Rha]⁺ 415.3 [M+H-2Glu-2Rha]⁺ 397.3 [M+H-2Glu-2Rha-H₂O]⁺	1029.5	pseudoprotodioscin
7	5.52	885.5	739.4 [M+H-Rha]⁺ 577.4 [M+H-Glu-Rha]⁺ 415.3 [M+H-2Glu-Rha]⁺ 397.3 [M+H-2Glu-Rha-H₂O]⁺	883.5	26-O-β-D-glucopyranosyl-3β,26-diol-25(R)-furost-5,20(22)-dien-3-O-α-L-rhamnopyranosyl(1→2)-O-β-D-glucopyranoside
8	9.1	1069.5 [M+Na]⁺	885.4789 [M+H-Glu]⁺ 577.37 [M+H-2Glu-Rha]⁺ 415.32 [M+H-3Glu-Rha]⁺ 397.31 [M+H-3Glu-Rha-H₂O]⁺ 271.20 [M+H-3Glu-Rha-C₈H₁₆O₂-H₂O]⁺	1045.5	zingiberensis saponin I
9	9.39	885.5	739.43 [M+H-Rha]⁺ 577.3696 [M+H-Glu-Rha]⁺ 415.3174 [M+H-2Glu-Rha]⁺ 397.3060 [M+H-2Glu-Rha-H₂O]⁺	883.5	deltonin
10	9.51	869.5	723.43 [M+H-Rha]⁺ 577.37 [M+H-2Rha]⁺ 415.32 [M+H-Glu-2Rha]⁺ 271.20 [M+H-Glu-2Rha-C₈H₁₆O₂]⁺ 253.19 [M+H-Glu-2Rha-C₈H₁₆O₂-H₂O]⁺	913.5 [M+HCOO]⁻	dioscin
11	9.83	723.4	577.37 [M+H-Rha]⁺ 415.32 [M+H-Glu-Rha]⁺ 397.31 [M+H-Glu-Rha-H₂O]⁺ 271.20 [M+H-Glu-Rha-C₈H₁₆O₂]⁺ 253.19 [M+H-Glu-Rha-C₈H₁₆O₂-H₂O]⁺	721.4 767.4 [M+HCOO]⁻	progenin III
12	13.39	415.3	271.20 [M+H-C₈H₁₆O₂]⁺ 253.19 [M+H-H₂O-C₈H₁₆O₂]⁺	-	diosgenin