Fig. 6.
Mice with excised bGH 3′UTR have normal-sized hearts. A: Gene targeting design indicating the location of loxP sites flanking the bovine growth hormone (bGH) 3′UTR segment. B: Admhi/+ mice were bred to the CMV-Cre line to globally excise the bGH 3′UTR fragment from the Admhi allele. This new allele is designated as Adm3′Δ. After removing the Cre recombinase by means of breeding, Adm expression was assessed by qRT-PCR (n = 5–6). C: At 2–3 months of age, the heart weight to body weight (HW:BW) ratios of Adm3′Δ/3′Δ mice was compared with the HW:BW ratios of age-matched Admhi/hi and wild-type mice (n = 5–7). All values represent means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001