FIGURE 4.

Uptake of HPLC-purified ¹⁸F-FASu in EL4 and SKOV-3 cells in vitro. (A) EL4 cells were evaluated for uptake of ³H-l-ASu and ¹⁸F-FASu in absence or presence of 1 mM l-Glu or l-ASu for 30 min. Uptake and inhibition profiles were similar for both radiolabeled compounds. Values are normalized to uptake of each agent in phosphate-buffered-saline-alone control (mean ± SD, n = 4). (B) ¹⁸F-FASu uptake in SKOV-3 cells in absence and presence of 0.5 mM sulfasalazine was evaluated with 5-, 20-, 40-, and 60-min incubations. Uptake values are raw CPM values per sample (mean ± SD, n = 3). SSZ = sulfasalazine.