Table 11.

IC50 values for inhibition of luciferase refolding and proliferation of MCF7 cells.

Compound	IC50 luciferase refolding (µM) 1	IC50 MCF7 proliferation (µM) 2
Anthothecol	12 ± 2	0.5 ± 0.06
Rottlerin	63 ± 5	7 ± 3
Garcinol	12 ± 5	4 ± 0.6
Piplartine	80 ± 7	10 ± 3

¹ Anthothecol, rottlerin, garcinol and piplartine were titrated into wells containing denatured luciferase and reticulocyte lysate. After a two-hour incubation period, assay buffer containing luciferin was added, and relative luminescence was measured. IC₅₀ is the concentration of the compound that inhibited luciferase refolding by 50% compared to the DMSO control; ² MCF-7 cells were treated in culture with anthothecol, rottlerin, garcinol and piplartine and DMSO as a control. Proliferation was assessed at 48 h using an MTS assay. Proliferation is defined as the colorimetric intensity difference between wells treated with DMSO and wells treated with the compounds.