Figure 1.

A diagram showing the approximate positions of the primer annealing sites (not to scale). Sequence derived from the transformation construct is shown in red, and barley genomic sequence is shown in green. The regions referred to as “junction sequence” and “flanking sequence” are labelled. The TSP1, TSP2 and TSP3 primers were used for junction sequence amplification. TSP3 was then used in combination with an FS_r primer to verify the junction sequence obtained for each line. The FS_f and FS_r primers were used in combination to amplify genomic DNA within untransformed Golden Promise, to confirm that the flanking sequence obtained derived from barley.