Figure 1.

Adherence of L. crispatus ST1 and binding of His₆-GS of ST1 are affected by pH. (A) Adherence of L. crispatus ST1 at pH 4 and pH 8 to the immobilized basement membrane preparation Matrigel and to BSA. Prior to the assays, the bacteria from an overnight culture were washed with the pH 4 or the pH 8 buffer, and the adhesion assays were performed at the corresponding pH. Means of adherent bacteria in 20 randomly chosen microscopic fields of 1.6 × 10⁴ μm² and standard deviations are shown. (B) Binding of purified His₆-GS to immobilized laminin was tested by surface plasmon resonance at pH 5.5, 6.0 and 6.5 (Adapted from [28] Copyright^© American Society for Microbiology).