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Comparison of the squared fluctuations predicted by ANM mode 2 of the NVP bound 1VRT (red) and EFV bound 1IKW structures (black). The main difference between the two structures is in the extent of the predicted mobility of the p66 thumb (residues 244 to 322); in both cases this is much than that observed for 1RTH and 1FK9 (see Figure 5).
