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. 2013 Nov 28;2(4):1378–1410. doi: 10.3390/biology2041378

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Rabl chromosome organization in wheat root tissue. (a) Centromeres (green) and telomeres (red) are labelled by fluorescence in situ hybridization (FISH) and are located at opposite sides of the nuclei; (b) Diagrammatic interpretation of the organization in (a); (c) Introgressed pair of rye chromosomes in wheat labelled by genomic in situ hybridization (GISH) with total rye genomic probe confirms a Rabl organization of individual CTs. (d) Single rye arm translocation into wheat localized by GISH. In (c) and (d) the RH panel in each case shows a nucleus from a seedling treated with 5-AC, whereas the LH panel shows a control untreated seedling. The 5-AC treatment has disrupted the CT organization, but the CTs remain in a Rabl configuration. Bar = 10 µm.