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. 2013 Nov 28;2(4):1378–1410. doi: 10.3390/biology2041378

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Vernalization in Arabidopsis involves Polycomb-mediated epigenetic silencing of FLOWERING LOCUS C (FLC) and physical clustering of FLC alleles. (a) Schematic representation of changes in FLC expression during vernalization; (b) FLC nuclear position was monitored in root nuclei using a FLC-lacO transgene. The lacO array was inserted downstream of the polyadenylation site of FLC; (c–d) Representative fluorescence images of Arabidopsis root cells in plants grown in warmth conditions (non-vernalized, NV) (c) and plants grown in cold for 2 weeks (vernalized, +V) (d). (scale bars: 5 μm).